Week 2: Unknown Bacteria S8772274699M
Materials and Methods
The next step was to perform the oxidase test. Initially, the bacteria sample was inconclusive. The oxidase check was fulfilled by using the pure culture plate along with using the broth. The same process was conducted on 9/11/18 to retest, and the results came back oxidase negative. I proceeded to the sugar fermentation test. By using a loop to obtain a small number of bacteria and added it to lactose and glucose. These were then placed in the incubator at 37.9℃ for five days. Results showed that the bacteria was negative for carbohydrate fermentation (lactose) but showed positive for gas and fermentation (glucose). The first phase of the SIM test was carried out. The isolated bacterial colonies were positioned into the SIM medium. Then placed into the incubator set at 37.3℃ for 24 hours. The SIM test showed positive for motility. I will be continuing to the next phase of the project which is gelatin testing. Those results will be known next week and should tell me what type of unknown bacteria S8772274699M is.
Oxidase Test
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Sugar Fermentation
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SIM Test
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Swab
Oxidase
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Lactose
Glucose
Durham tube
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SIM
Needle
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| Lactose Fermentation |
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| Glucose Fermentation |
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| SIM Test |
Finding the unknown bacteria is such a fun project and it gives such a great introduction to microbiology. Good luck with the rest of the tests and finding out which bacteria you have.
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